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    1. 聯(lián) 系 人:莫經(jīng)理

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      產(chǎn)品展示

      1%豚鼠醛化細胞懸液(無(wú)菌)

      1%豚鼠醛化細胞懸液(無(wú)菌)

      價(jià)格:電議 型號:

      原產(chǎn)地:中國大陸發(fā)布時(shí)間:2024/3/25 16:04:40

      1%豚鼠醛化細胞懸液(無(wú)菌),紅細胞醛化后沉積細胞體 積約增加20%,紅細胞固有形態(tài)無(wú)變化,呈棕色,在有Na+存在時(shí)顏色略紅。

      采購度:162

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      詳細內容

      1%豚鼠醛化細胞懸液(無(wú)菌)

      產(chǎn)品名稱(chēng)】 :1%豚鼠醛化細胞懸液

      貨號】:FXQ364-01

      產(chǎn)品規格】 :100ml                            

      儲存條件】 :2~8℃                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
      有效期】 :三個(gè)月
       
      醛化紅細胞特點(diǎn):
      1.醛化紅細胞鏡檢時(shí)一般呈兩面凸起的園盤(pán)狀,散在,不聚集成團。
      2.紅細胞醛化后沉積細胞體 積約增加20%,紅細胞固有形態(tài)無(wú)變化,呈棕色,在有Na+存在時(shí)顏色略紅。
      3.醛化細胞具有較強的抗破壞能力,反復凍融,加熱至60,蒸餾水中低滲均不破壞,凍干后重新以水懸浮并無(wú)明 顯變化,pH<3.0時(shí),可自然凝集,但pH達1.8時(shí)也不溶血;pH≥10.2可有中度溶血。醛化細胞懸液4可存放3個(gè)月以上,但凍結后重新融化則凝集成團。
       
      戊二醛化紅細胞制備法:
      沉積紅細胞用10~20倍0.11MpH7.2 PB洗4~6,再次配成10%懸液,取此懸液 (預先冷至4) 緩慢地加入到等量 1%的戊二醛(25%戊二醛用0.11MpH7.2PB稀釋 之)中,并繼續搖動(dòng)30~60分鐘,然后用蒸餾水洗五次。*后用重蒸餾水 配成20%的懸液,加0.1%NaN3防腐,4冰箱保存備用。
      丙酮醛一一甲醛化紅細胞制備:
      (1)沉積紅細胞以10~20倍體積 0.11MpH7.2PB洗滌4~6次,然后用該PB配成8%紅細胞懸液。
      (2)8%紅細胞懸液中,緩慢加入等量3%丙酮醛、3%甲醛PB液(取丙酮醛15ml、甲醛8ml、0.11MpH7.2PB60ml,用10%Na-OH調正pH至7.2再加同樣PB至100mI)于20左右緩慢攪拌17小時(shí),至時(shí)用0.11MpH7.2PB洗三次,并用同樣PB制成20%紅細胞懸液,加入0.1%NaN3防腐,4冰箱保存備用。
      注意事項:
      1.在加醛類(lèi)前要盡量將紅細胞洗凈;
      2.開(kāi)始加入甲醛濃度要淡,以后逐漸提高甲醛濃度,否則紅細胞容易變形;
      3.作適當的搖動(dòng),使甲醛與紅細胞充分接觸。
       
      相關(guān)產(chǎn)品如下:
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      100ml
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      FXQ364-08
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      FXQ364-10
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      FXQ364-15
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       1%豚鼠醛化細胞懸液(無(wú)菌)

      更新時(shí)間:2024/6/25 16:42:51

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